Occurrence and antimicrobial resistance of Campylobacter jejuni isolates from poultry in Casablanca-Settat, Morocco

  • Khadija Es-soucratti | khadijaessoucratti@gmail.com Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Hassan II University of Casablanca; Laboratory of Microbiology, Department of Food Hygiene, Products and Environment, Institut Pasteur du Maroc, Casablanca, Morocco.
  • Abderrahman Hammoumi Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Hassan II University of Casablanca, Morocco.
  • Brahim Bouchrif Laboratory of Microbiology, Department of Food Hygiene, Products and Environment, Institut Pasteur du Maroc, Casablanca, Morocco.
  • Rajaa Asmai Laboratory of Microbiology, Department of Food Hygiene, Products and Environment, Institut Pasteur du Maroc, Casablanca, Morocco.
  • Houda En-nassiri Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Hassan II University of Casablanca; Laboratory of Microbiology, Department of Food Hygiene, Products and Environment, Institut Pasteur du Maroc, Casablanca, Morocco.
  • Bouchra Karraouan Laboratory of Microbiology, Department of Food Hygiene, Products and Environment, Institut Pasteur du Maroc, Casablanca, Morocco.

Abstract

Campylobacteriosis and Campylobacter spp. resistance to antibiotics represents a serious worldwide public health problem thermophilic Campylobacters, in particular, are major causes of gastroenteritis in humans. The aim of this study was to determine the prevalence and antimicrobial resistance of Campylobacter jejuni isolated from chicken droppings, of commercial poultry in the city of Casablanca, Morocco. Between February and September 2017, 140 samples of chicken droppings were collected and analyzed by classical bacteriology methods for isolation and identification according to Moroccan Standard NM ISO/TS 10272-3 (2013), followed by molecular identification (PCR: polymerase chain reaction). Among the 140 samples, 102 (73%) were positive by Campylobacter spp. tests and 38 (27.14 %) were negative to Campylobacter spp. Among the positive colonies, 41 (40, 2%) were C. jejuni. Of the 41 C. jejuni isolates, resistance was detected to tetracycline (100%), erythromycin (97%), ampicillin (85%), ciprofloxacin (77%), amoxicillin/ clavulanic acid (61.4%), and gentamicin (12.0%). In conclusion, the data obtained in the current study demonstrate that the majority of C. jejuni isolates evaluated were resistant to antimicrobials of the cycline, macrolide, and fluoroquinolone families, and all of the isolates were susceptible to gentamicin. Fluoroquinolone is the drug of choice for treating Campylobacter infections. These results underline the need for prudent use of antibiotics in poultry production to minimize the spread of antibioticresistant Campylobacter spp.

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Published
2020-04-06
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Original Articles
Keywords:
Campylobacter spp,, Chicken droppings, Market, Morocco, Antimicrobial resistance, Molecular identification (PCR)
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How to Cite
Es-soucratti, K., Hammoumi, A., Bouchrif, B., Asmai, R., En-nassiri, H., & Karraouan, B. (2020). Occurrence and antimicrobial resistance of Campylobacter jejuni isolates from poultry in Casablanca-Settat, Morocco. Italian Journal of Food Safety, 9(1). https://doi.org/10.4081/ijfs.2020.8692