Application of the Micro Biological Survey analytical method for the determination of bacterial load in cow raw milk


https://doi.org/10.4081/ijfs.2020.7696
Vol. 9 No. 3 (2020)
Submitted: 15 July 2018
Accepted: 18 January 2019
Published: 4 November 2020
Plate count at 30°C (per ml), MBS test, Flow citometry, Cow raw milk, Bactoscan FC
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Authors

  • Alessandra Cornacchia
    a.cornacchia@izs.it
    Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Maria Antonietta Saletti Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Violeta Di Marzio Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Romolo Salini Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Cristina Marfoglia Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Elga Tieri Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Nicola D’Alterio Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Nicla Marri Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, Rome, Italy.
  • Francesca Losito Istituto Nazionale Biostrutture e Biosistemi – Consorzio Interuniversitario, Rome, Italy.
  • Alyexandra Arienzo Dipartimento di Scienze, Università degli Studi Roma Tre, Rome, Italy.
  • Lorenza Murgia Dipartimento di Scienze, Università degli Studi Roma Tre, Rome, Italy.
  • Giovanni Antonini Istituto Nazionale Biostrutture e Biosistemi – Consorzio Interuniversitario, Rome; Dipartimento di Scienze, Università degli Studi Roma Tre, Rome, Italy.
  • Simonetta Amatiste Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, Rome, Italy.
  • Loris Leboffe Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, Rome; Dipartimento di Scienze, Università degli Studi Roma Tre, Rome, Italy.
  • Francesco Pomilio Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.

The aim of this study was to evaluate the performance of “Micro Biological Survey – MBS Test” in the enumeration of bacterial load in cow raw milk. The MBS test is based on a colorimetric method recently developed and patented by “Roma Tre” University, Italy. The evaluation of the performance of the MBS method was carried out by comparison with plate count at 30°C (gold standard) and flow cytometry. Thirteen independent set of experiments were performed analyzing a total of 104 samples of cow raw milk with the selected methods. Results obtained using the MBS method are comparable with those obtained with the plate count method at 30°C (CFU/mL) and flow cytometry technology; in particular, the results obtained with the MBS method are very close to plate count’s at 30°C. On the other hand, there are statistically significant differences between these two methods’ and flow cytometry technology’s results that could be due to the different experimental conditions.


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1.
Cornacchia A, Saletti MA, Di Marzio V, Salini R, Marfoglia C, Tieri E, D’Alterio N, Marri N, Losito F, Arienzo A, Murgia L, Antonini G, Amatiste S, Leboffe L, Pomilio F. Application of the Micro Biological Survey analytical method for the determination of bacterial load in cow raw milk. Ital J Food Safety [Internet]. 2020 Nov. 4 [cited 2024 Apr. 19];9(3). Available from: https://www.pagepressjournals.org/ijfs/article/view/7696

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