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Evaluation of post-fermentation heating times and temperatures for controlling Shiga toxin-producing Escherichia coli cells in a non-dried, pepperoni-type sausage

Laura E. Shane, Anna C.S. Porto-Fett, Bradley A. Shoyer, Randall K. Phebus, Harshavardhan Thippareddi, Ashley Hallowell, Kelsey Miller, Lianna Foster-Bey, Stephen G. Campano, Peter J. Taormina, Daniel L. Glowski, Robert B. Tompkin, John B. Luchansky
  • Laura E. Shane
    Delaware Valley University, PA; United States Department of Agriculture, Agricultural Research Service, PA, United States
  • Anna C.S. Porto-Fett
    United States Department of Agriculture, Agricultural Research Service, PA, United States
  • Bradley A. Shoyer
    United States Department of Agriculture, Agricultural Research Service, PA, United States
  • Randall K. Phebus
    Kansas State University, KS, United States
  • Harshavardhan Thippareddi
    University of Nebraska, NE, United States
  • Ashley Hallowell
    United States Department of Agriculture, Agricultural Research Service, PA, United States
  • Kelsey Miller
    Ursinus College, PA, United States
  • Lianna Foster-Bey
    Ursinus College, PA, United States
  • Stephen G. Campano
    Hawkins Inc., MN, United States
  • Peter J. Taormina
    John Morrell Food Group, IL, United States
  • Daniel L. Glowski
    John Morrell Food Group, IL, United States
  • Robert B. Tompkin
    LaGrange Highlands, IL, United States
  • John B. Luchansky
    United States Department of Agriculture, Agricultural Research Service, PA, United States | john.luchansky@ars.usda.gov

Abstract

Coarse ground meat was mixed with non-meat ingredients and starter culture (Pediococcus acidilactici) and then inoculated with an 8-strain cocktail of Shiga toxinproducing Escherichia coli (ca. 7.0 log CFU/g). Batter was fine ground, stuffed into fibrous casings, and fermented at 35.6°C and ca. 85% RH to a final target pH of ca. pH 4.6 or ca. pH 5.0. After fermentation, the pepperoni- like sausage were heated to target internal temperatures of 37.8°, 43.3°, 48.9°, and 54.4°C and held for 0.5 to 12.5 h. Regardless of the heating temperature, the endpoint pH in products fermented to a target pH of pH 4.6 and pH 5.0 was pH 4.56±0.13 (range of pH 4.20 to pH 4.86) and pH 4.96±0.12 (range of pH 4.70 to pH 5.21), respectively. Fermentation alone delivered ca. a 0.3- to 1.2-log CFU/g reduction in pathogen numbers. Fermentation to ca. pH 4.6 or ca. pH 5.0 followed by post-fermentation heating to 37.8° to 54.4°C and holding for 0.5 to 12.5 h generated total reductions of ca. 2.0 to 6.7 log CFU/g.

Keywords

Shiga toxin-producing Escherichia coli, Fermentation, Food Safety, Thermal Inactivation, Heating.

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Submitted: 2017-12-21 21:42:43
Published: 2018-07-03 08:05:55
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Copyright (c) 2018 Laura E. Shane, Anna C.S. Porto-Fett, Bradley A. Shoyer, Randall K. Phebus, Harshavardhan Thippareddi, Ashley Hallowell, Kelsey Miller, Lianna Foster-Bey, Stephen G. Campano, Peter J. Taormina, Daniel L. Glowski, Robert B. Tompkin, John B. Luchansky

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