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Dissemination and persistence of Pseudomonas spp. in small-scale dairy farms

Daniele Michele Nucera, Sara Lomonaco, Patrizia Morra, Marco Francesco Ortoffi, Daniele Giaccone, Maria Ausilia Grassi
  • Sara Lomonaco
    Department of Veterinary Sciences, University of Turin, Grugliasco (TO), Italy
  • Patrizia Morra
    Department of Veterinary Sciences, University of Turin, Grugliasco (TO), Italy
  • Marco Francesco Ortoffi
    Department of Veterinary Sciences, University of Turin, Grugliasco (TO), Italy
  • Daniele Giaccone
    Regional Association of Farmers of Piedmont, Turin, Italy
  • Maria Ausilia Grassi
    Department of Veterinary Sciences, University of Turin, Grugliasco (TO), Italy

Abstract

This study was aimed at collecting data on presence, dissemination and persistence of Pseudomonas in small-scale dairy farms. Six farms (located in Piedmont) were visited three times over 2014: 116 waters (wells and different faucets/pipes) and 117 environmental samples (milking equipments and drains) were collected. Enumeration of Pseudomonadaceae was performed, 3-5 colonies/samples were selected for identification via 16SrDNA/oprI polymerase chain reaction (PCR), and typed by enterobacterial-repetitive- intergenic-consensus (ERIC)-PCR. Pseudomonadaceae were detected in 77% of samples. No statistical differences were found among proportions of positives across farms, sample typologies and seasons. Most isolates were Pseudomonas fluorescens (45%), and ERIC-PCR showed 32 persistent types diffused across farms. All in all, Pseudomonas spp. represents a challenge, considering its presence over time in water as well as in teat cups, indicating a continuous source of contamination. Moreover, persistency of strains may indicate biofilm-formation and/or sanitisers resistance, therefore emphasising the role of primary production for preventing milk contamination by Pseudomonas spp.

Keywords

Pseudomonas; Small-scale dairy farms; Water; Typing; Persistence

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Submitted: 2015-11-29 21:41:23
Published: 2016-05-02 15:14:27
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Copyright (c) 2016 Daniele Michele Nucera, Sara Lomonaco, Patrizia Morra, Marco Francesco Ortoffi, Daniele Giaccone, Maria Ausilia Grassi

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