Incremental detection of pulmonary tuberculosis among presumptive patients by GeneXpert MTB/RIF® over fluorescent microscopy in Mwanza, Tanzania: an operational study


https://doi.org/10.4081/hls.2015.5011
Vol. 3 No. 1 (2015)
Submitted: 17 January 2015
Accepted: 26 March 2015
Published: 26 June 2015
Tuberculosis detection, GeneXpert MTB/RIF®, Mwanza, Tanzania
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Authors

  • Jeremiah Seni
    senijj80@gmail.com
    Department of Microbiology and Immunology, Catholic University of Health and Allied Sciences Bugando, Mwanza, Tanzania, United Republic of.
  • Benson R. Kidenya Department of Microbiology and Immunology, Catholic University of Health and Allied Sciences Bugando, Mwanza, Tanzania, United Republic of.
  • Mercy Anga Department of Microbiology and Immunology, Catholic University of Health and Allied Sciences Bugando, Mwanza, Tanzania, United Republic of.
  • Anthony Kapesa Department of Microbiology and Immunology, Catholic University of Health and Allied Sciences Bugando, Mwanza, Tanzania, United Republic of.
  • John R. Meda Department of Internal Medicine, University of Dodoma, Tanzania, United Republic of.
  • Richard Mutakyawa Sekou Toure Regional Referral Hospital, Mwanza; PATH - Tanzania, Dar es Salaam, Tanzania, United Republic of.
  • Zahra H. Mkomwa PATH - Tanzania, Dar es Salaam, Tanzania, United Republic of.
  • Fidelis Marcel Sekou Toure Regional Referral Hospital, Mwanza, Tanzania, United Republic of.
  • John M. Changalucha National Institute for Medical Research, Mwanza Medical Research Centre, Tanzania, United Republic of.
  • Stephen E. Mshana Department of Microbiology and Immunology, Catholic University of Health and Allied Sciences Bugando, Mwanza, Tanzania, United Republic of.
Laboratory confirmation among presumptive tuberculosis (PTB) patients is pivotal in ensuring prompt management. Limited information exists in Tanzania regarding the performance of GeneXpert MTB/RIF® in comparison with conventional methods. An operational study was conducted involving 806 PTB patients at Sekou Toure Hospital in Mwanza, Tanzania from June to November 2013. Patients’ information was obtained and their respective sputum samples analyzed by lightemitting diode fluorescent microscopy (LED FM) and GeneXpert MTB/RIF®. The mean age of study participants was 39.6±16.0 years, with males accounting for 50.5%. The majority of patients (97.5%) were new cases. The proportions of PTB patients confirmed by LED FM and GeneXpert MTB/RIF® were 14.1% (114/806) and 23.7% (191/806) respectively, resulting into a 9.6% incremental detection rate by GeneXpert MTB/RIF® over LED FM. The detection rate among HIV positive individuals was also higher [23.6% (63/267) vs 14.2% (38/267), respectively], with an incremental detection of 9.4%. The incremental detection of PTB by GeneXpert MTB/RIF® over LED FM calls for expansion of its use to increase detection of smear negative PTB among people living with HIV.

Jeremiah Seni, Department of Microbiology and Immunology, Catholic University of Health and Allied Sciences Bugando, Mwanza
Lecturer & Head of Department of Microbiology and Immunology

Supporting Agencies

PATH Tanzania under USAID TB TO 2015

Seni, J., Kidenya, B. R., Anga, M., Kapesa, A., Meda, J. R., Mutakyawa, R., Mkomwa, Z. H., Marcel, F., Changalucha, J. M., & Mshana, S. E. (2015). Incremental detection of pulmonary tuberculosis among presumptive patients by GeneXpert MTB/RIF® over fluorescent microscopy in Mwanza, Tanzania: an operational study. Healthcare in Low-Resource Settings, 3(1). https://doi.org/10.4081/hls.2015.5011

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