Advances in Oceanography and Limnology 2020-05-30T20:51:31+00:00 Nadia Moscato Open Journal Systems <p><strong>Advances in Oceanography and Limnology</strong>&nbsp;(<em>AIOL Journal</em>) is the official publication of the <a href="" target="_blank" rel="noopener">Italian Association of Limnology and Oceanology</a> (A.I.O.L.).</p> <p>The <em>AIOL Journal </em>publishes original research articles and reviews on different topics and novel discoveries in the fields of limnology and oceanography. Papers may deal with different or single physical, chemical and biological aspects, including biomolecules, populations and communities, ecosystem functioning and interactions between global change and ecosystems. Environmental monitoring and studies of regional importance will be considered only if they contribute to the general advance of aquatic sciences. Multidisciplinary articles linking different scientific disciplines (e.g., community ecology and metabolomic/toxicology, ecology and phylogenetic, water quality and economy…) are equally considered. Particularly welcomed are studies focusing on marine and freshwater ecosystems.</p> <p>Two regular issues of the <strong>Advances in Oceanography and Limnology</strong> are published each year. In addition, Special Issues and Proceedings that focus on topics that are timely and of interest to a significant number of aquatic scientists are published. From 2010 to 2014, previous issues of the <em>AIOL Journal</em> have been published by&nbsp;<a href="">T&amp;F</a>.</p> <p>This journal does not apply charge for publication to Authors as it is supported by institutional funds.</p> Isolation and identification of halophilic and halotolerant bacteria from the sediments of the Qeshm Island mangrove forest 2020-05-30T20:51:31+00:00 Pegah Javid Hassan Zadabbas Shahabadi Homeyra Amirkhani Narges Amrollahi Mohammad Sharif Ranjbar <p>Due to specific environmental and ecological conditions, mangrove forests are known as marine transitional zones between sea and land, and, as such, they host organisms with high ecological plasticity. The mangrove forests of Qeshm Island (Iran) are relatively pristine habitats and represent an ideal target for investigating patterns of either aquatic or benthic biodiversity. To provide insights on microbial diversity in this area, nineteen halophilic and halotolerant bacteria were isolated from the sediments in 2017 during low tide. The extracted bacterial strains were studied morphologically by streaking, initial observation of colonies and bacterial staining, and characterized using a battery of biochemical tests including KOH, MR, VP, urease, TSI, S/I/M, Mac, LIA, ODC, ADH, oxidase, catalase, and tryptophan deaminase. The optimum growth of halophilic bacteria was observed in salt concentrations from 5 to 20% NaCl, whereas the extreme halophilic Gram-positive strain grew in salt concentration of up to 30% NaCl. Molecular analyses were also carried out on four halophilic strains and one extreme halophilic gram-positive bacteria. Phylogenetic taxonomy analysis, after 16S rDNA gene Sanger sequencing, revealed that the halophilic bacteria were closely related to the strain types of the genus <em>Bacillus</em> including <em>Bacillus licheniformis</em>, <em>Bacillus velezensis</em>, <em>Bacillus Paralicheniformis </em>and <em>Bacillus</em> sp. with 99% bootstrap value. The extreme halophilic strain was associated to strains of <em>Planococcus plakortidis</em> with 100% bootstrap value.</p> 2020-05-12T00:00:00+00:00 ##submission.copyrightStatement## Co-occurrence of anatoxin-a and microcystins in Lake Garda and other deep subalpine lakes 2020-05-30T20:51:28+00:00 Leonardo Cerasino Nico Salmaso <p>Cyanotoxins are a global concern in freshwaters and eutrophication and climate changes can have synergistic effects in exacerbating the problem. Deep Subalpine Lakes (DSL) are a group of lakes of huge economic and naturalistic importance in Italy. Together with eutrophication (occurred during 1960s and 1970s) and re-oligotrophication (from 1990 onward) these lake have been experiencing warming and increase of the water column stability. These changes have influenced the phytoplankton (comprised the cyanobacteria) community of the lakes. Four DSL lakes (Lakes Garda, Iseo, Como and Lugano) have been studied with the aim of comparing their toxic potential. For one of them (Lake Garda) an 8 years survey was conducted, allowing a long term trend analysis. Toxin analysis was conducted on a monthly basis by targeted LC-MS/MS. A screening for anatoxins, cylindrospermopsins, saxitoxins, microcystins (MCs) and nodularins was carried out. Among all the listed toxins, only one anatoxin and five MCs were detected in the lakes. In particular, the alkaloid Anatoxin-a (ATX) was found dominant Lakes Garda, Iseo and Como and absent in lake Lugano; the MC-[D-Asp3]RR was found as the most abundant MC in all four lakes. Four other minoritary MCs were also found. The two major toxins are produced by two different cyanobacteria (<em>Tychonema bourrellyi</em> and <em>Planktothrix rubescens</em>) which have however similar ecological traits. Peaks of these toxins occur in warmer months (typically between May and September) in the thermocline layer (around 20 m, in the considered lakes). In summer 2016, the highest concentrations of ATX and total microcystins (MCs) were registered in Lake Iseo (1100 and 430ngL<sup>-1</sup>, respectively), while in the other lakes values were approximately twice lower. In the lakes where it is present, ATX peak levels are much higher than MCs, thus highlighting the necessity of considering ATX in the procedures of risk assessment. The importance of ATX is expected to further grow in the future with respect to MCs, as demonstrated by the long term trend analysis carried out in Lake Garda that showed a clear decline for MCs from 2009 till 2016 and a relative constancy for ATX.</p> 2020-05-12T12:53:31+00:00 ##submission.copyrightStatement##