Exploring the world of micro sculptures - subfossil Cladocera remains under the SEM

  • Izabela Zawiska | izawiska@twarda.pan.pl Polish Academy of Sciences, Department of Geoecology and Climatology, Poland.
  • Edyta Zawisza Polish Academy of Sciences, Institute of Geological Sciences, Poland.
  • Marta Wojewódka Polish Academy of Sciences, Institute of Geological Sciences, Poland.
  • Artem Y. Sinev Lomonosov Moscow State University, Department of Invertebrate Zoology, Russian Federation.

Abstract

The scanning electron microscope (SEM) is widely used for the identification of microstructural characteristics and morphology of different microorganisms. Common procedures are based and developed for remains of living species. This paper presents an effective method for drying and preparing subfossil Cladocera remains for SEM observation, which has been recently adapted and tested on several samples originating from different American and European lakes. This method results to be fast and cheap, as it excludes the use of expensive and toxic reagents. Moreover, it allows to recognize the micro sculpture and other species specific characteristics present on the different body parts of the Cladocera remains. The present contribution provides 29 high quality pictures of 12 cladoceran species at magnification between 200x and 11,000x. SEM images reveal  that the patterns observed on the shells under the light microscope actually are always three dimensional structures.

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Published
2016-12-29
Info
Issue
Section
Thematic papers - Subfossil Cladocera
Supporting Agencies
Polish National Science Centre, EEA and Norway Grants, Institute of Geography and Spatial Organization Polish Academy of Sciences, Institute of Geological Sciences, Polish Academy of Sciences.
Keywords:
SEM, subfossil Cladocera, micro sculpture, chitin.
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How to Cite
Zawiska, I., Zawisza, E., Wojewódka, M., & Sinev, A. Y. (2016). Exploring the world of micro sculptures - subfossil Cladocera remains under the SEM. Advances in Oceanography and Limnology, 7(2). https://doi.org/10.4081/aiol.2016.6218

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