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Microcystin-LR acute exposure does not alter in vitro and in vivo ATP, ADP and AMP hydrolysis in adult zebrafish (Danio rerio) brain membranes

Luiza Wilges Kist, Rachel Seemann Fritsch, João Sarkis Yunes, Carla Denise Bonan, Maurício Reis Bogo
  • Luiza Wilges Kist
    Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS; Instituto Nacional de Ciência e Tecnologia Translacional em Medicina (INCT-TM), Porto Alegre, RS, Brazil
  • Rachel Seemann Fritsch
    Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil
  • João Sarkis Yunes
    Unidade de Pesquisas em Cianobactérias, Prédio da Hidroquímica, Instituto de Oceanografia, Campus Carreiros da FURG, Brazil
  • Carla Denise Bonan
    Instituto Nacional de Ciência e Tecnologia Translacional em Medicina (INCT-TM), Porto Alegre, RS; Laboratório de Neuroquímica e Psicofarmacologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil
  • Maurício Reis Bogo
    Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS; Instituto Nacional de Ciência e Tecnologia Translacional em Medicina (INCT-TM), Porto Alegre, RS, Brazil | mbogo@pucrs.br

Abstract

Microcystins (MCs) are toxins produced by cyanobacteria during the blooms that could accumulate in aquatic animals and be relocated to higher trophic levels. Adenosine triphosphate (ATP) acts as an excitatory neurotransmitter and/or a neuromodulator in the extracellular space playing important roles in physiological and pathological conditions. The aim of this study was, therefore, to evaluate the acute effects of different concentrations of MC-LR on nucleoside triphosphate diphosphohydrolases and 5’-nucleotidade in adult zebrafish (Danio rerio) brain membranes. The results have shown no significant changes in ATP, adenosine diphosphate (ADP) and adenosine monophosphate (AMP) hydrolysis in zebrafish brain membranes. MC-LR in vitro also did not alter ATP, ADP and AMP hydrolysis in the concentrations tested. These findings show that acute exposure to MC-LR did not modulate ectonucleotidase activity in the conditions tested. However, additional studies including chronic exposure should be performed in order to achieve a better understanding about MC-LR toxicity mechanisms in the central nervous system.

Keywords

microcystin-LR, zebrafish, ectonucleotidases, neurotoxicity.

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Submitted: 2012-07-05 16:09:10
Published: 2012-10-03 15:56:00
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Copyright (c) 2012 Luiza Wilges Kist, Rachel Seemann Fritsch, João Sarkis Yunes, Carla Denise Bonan, Maurício Reis Bogo

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