Suspected chromosomally integrated human herpes virus 6 in hematopoietic stem cell transplantation
Submitted: 19 July 2014
Accepted: 6 February 2016
Published: 31 March 2016
Accepted: 6 February 2016
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Materials and Methods: HHV-6 DNA extraction was performed by automated extractor and DNA was amplified-quantified by Real Time polymerase chain reaction. Species identification was performed by sequencing HHV6-U100 glycoprotein using automated sequencer and sequencing products were analysed using the Blast program.
Results: After autologous transplantation HHV6-DNAemia was 5.4 log copies/mL setting to 3.9 log copies/mL for a long period post allogenic transplantation. The patient’s hair follicles were tested for HHV- 6 DNA having positive results. Sequences of both strains of HHV6 extracts from blood and hair follicles resulted species B. HHV6 viral load decreased significantly after Lymphocyte Infusion by ci-HHV6 negative donor (sister), having steady viral load during the following six months of monitoring. One year later, patient is in complete haematological remission.
Conclusions: Detection of HHV-6 in hair follicles and HHV-6 DNAemia persistently elevated before allogenic transplant, confirm the occurrence of ci-HHV-6. The observed important decreasing viral load is potentially due to the successful engraftment of ci-HHV-6-negative donor marrow after allogeneic transplant.
Todisco, A., Landi, M., Festa, B. P., Santoro, L., Storti, G., Campanini, G., Ariola, R., Romeo, F., & Violano, G. (2016). Suspected chromosomally integrated human herpes virus 6 in hematopoietic stem cell transplantation. Microbiologia Medica, 31(1). https://doi.org/10.4081/mm.2016.4590
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