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Ultrasensitive MAPK/Erk activation in absence of protein synthesis in Xenopus oocytes

Rémy Beaujois, Franck Riquet, Katia Cailliau, Edith Browaeys-Poly, Christophe Russo, Ralf Blossey, Dorothée Vicogne, Matthieu Marin, Arlette Lescuyer-Rousseau, Jean-Pierre Vilain, Jean-François Bodart
  • Rémy Beaujois
    EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1; USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France | jean-francois.bodart@univ-lille1.fr
  • Franck Riquet
    EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1; USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France
  • Katia Cailliau
    EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France
  • Edith Browaeys-Poly
    EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France
  • Christophe Russo
    EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1; USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France
  • Ralf Blossey
    USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France
  • Dorothée Vicogne
    USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France
  • Matthieu Marin
    EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France
  • Arlette Lescuyer-Rousseau
    EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France
  • Jean-Pierre Vilain
    EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1, France
  • Jean-François Bodart
    EA 4479, Laboratoire de Régulation des Signaux de Division, University of Lille1; USR 3078 CNRS, Institut de Recherche Interdisciplinaire, Villeneuve d’Ascq, France

Abstract

The mitogen-activated protein kinase (MAPK) cascade in Xenopus oocytes exhibits an all-or-none, ultrasensitive response, which is believed to result from a positive feed-back loop. Here we describe a context where 1,10-phenanthroline slowly, but strongly, activates MAPK while it impairs protein synthesis in a zincdependent manner, abolishing any feed-back loop. The induced-MAPK response was found to be strongly ultrasensitive. We argue that underlying this behaviour is a regulation motif akin to a feed-forward loop acting in vivo.

Keywords

MAPK, ultrasensitivity, feed-back, protein synthesis, regulation motif.

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Submitted: 2012-09-17 18:28:45
Published: 2013-01-30 15:47:04
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Copyright (c) 2013 Rémy Beaujois, Franck Riquet, Katia Cailliau, Edith Browaeys-Poly, Christophe Russo, Ralf Blossey, Dorothée Vicogne, Matthieu Marin, Arlette Lescuyer-Rousseau, Jean-Pierre Vilain, Jean-François Bodart

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