Molecular typing of bacteria of the genus Asaia in malaria vector Anopheles arabiensis Patton, 1905


https://doi.org/10.4081/jear.2012.e7
Vol. 44 No. 2 (2012)
Submitted: 31 May 2012
Accepted: 19 July 2012
Published: 31 August 2012
Anopheles arabiensis, mosquito-associated bacteria, Asaia spp., molecular typing.
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Authors

  • S. Epis Scuola di Bioscienze e Biotecnologie, Università degli Studi di Camerino; DIVET, Università degli Studi di Milano, Italy.
  • M. Montagna DIVET, Università degli Studi di Milano, Italy.
  • F. Comandatore DIVET, Università degli Studi di Milano, Italy.
  • C. Damiani Scuola di Bioscienze e Biotecnologie, Università degli Studi di Camerino, Italy.
  • A. Diabaté Institut de Recherche en Science de Santé/Centre Muraz, Bobo-Dioulasso, Burkina Faso.
  • D. Daffonchio DEFENS, Università degli Studi di Milano, Italy.
  • B. Chouaia
    bessem.chouaia@unimi.it
    DEFENS, Università degli Studi di Milano, Italy.
  • G. Favia Scuola di Bioscienze e Biotecnologie, Università degli Studi di Camerino, Italy.
The acetic acid bacterium Asaia spp. was successfully detected in Anopheles arabiensis Patton, 1905, one of the major vector of human malaria in Sub-Saharan Africa. A collection of 45 Asaia isolates in cellfree media was established from 20 individuals collected from the field in Burkina Faso. 16S rRNA universal polymerase chain reaction (PCR) and specific qPCR, for the detection of Asaia spp. were performed in order to reveal the presence of different bacterial taxa associated with this insect. The isolates were typed by internal transcribed spacer-PCR, BOX-PCR, and randomly amplified polymorphic DNA-PCR, proved the presence of different Asaia in A. arabiensis.

Epis, S., Montagna, M., Comandatore, F., Damiani, C., Diabaté, A., Daffonchio, D., Chouaia, B., & Favia, G. (2012). Molecular typing of bacteria of the genus Asaia in malaria vector Anopheles arabiensis Patton, 1905. Journal of Entomological and Acarological Research, 44(2), e7. https://doi.org/10.4081/jear.2012.e7

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