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Evaluation of bovine beta casein polymorphism in two dairy farms located in northern Italy

Elisa Massella, Silvia Piva, Federica Giacometti, Gaetano Liuzzo, Angelo Vittorio Zambrini, Andrea Serraino
  • Elisa Massella
    Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy | elisa.massella3@unibo.it
  • Silvia Piva
    Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy
  • Federica Giacometti
    Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy
  • Gaetano Liuzzo
    A.U.S.L of Modena Carpi district, Carpi (MO), Italy
  • Angelo Vittorio Zambrini
    Department of Quality, Innovation, Safety, Environment, Granarolo S.p.A., Bologna, Italy
  • Andrea Serraino
    Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy

Abstract

Bovine beta casein A1 is one of the most common variants in dairy cattle breeds; it is considered a risk factor in milk intolerance and in other important human diseases, because of the bioactive peptide beta casomorphin-7 (BCM7) produced by raw or processed A1-milk, but not by A2- milk, during digestion. The aim of this study was to perform a cheap and rapid method to investigate beta casein polymorphism in copious animals. The study included 2 dairy farms with a totally of 1230 cows. Beta casein genotypes were estimated evaluating Exon 7 region of bovine beta casein gene (CSN2) by sequences analysis. In the population included in the study 5 variants (A1, A2, B, F, I) and 13 genotypes (A1A1, A1A2, A1B, A1F, A1I, A2A2, A2B, A2F, A2I, BB, BF, BI, FI) were detected. The method showed high sensibility and specificity, resulted low-cost and few time consuming.

Keywords

Dairy cows, Beta casein, Allelic variants, Sequencing

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Submitted: 2017-07-13 12:50:17
Published: 2017-09-29 11:00:18
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Copyright (c) 2017 Elisa Massella, Silvia Piva, Federica Giacometti, Gaetano Liuzzo, Angelo Vittorio Zambrini, Andrea Serraino

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